![]() A research article that demonstrates microfluidics-free monodispersed droplet generation with a vortexer. Particle-templated emulsification for microfluidics-free digital biology. passport book which we started issuing in 2021. An article describing the CITE-seq method. Sanger sequencing of individual specimens is the standard approach in generating large-scale DNA barcode libraries and identifying unknowns. DNA barcoding is a standardised and widely used method to identify specimens at the species level using a restricted set of short DNA fragmentsusually only the 5’ end of the cytochrome c oxidase subunit I (COI) in animals(Hebert et al. The ability to accurately characterize DNA variant proportions using PCR amplification is key to many genetic studies, including studying tumor heterogeneity, 16S microbiome, viral and immune receptor sequencing. Simultaneous epitope and transcriptome measurement in single cells. DNA barcoding is an efficient method to identify specimens and to detect undescribed/cryptic species. A research article that describes SPLiT-seq, a method that barcodes cellular RNA using a split pool directly on cells. Single-cell profiling of the developing mouse brain and spinal cord with split-pool barcoding. A research article that describes the 10x Genomics commercial scRNA-seq system. Massively parallel digital transcriptional profiling of single cells. A research article presenting a high-throughput scRNA-seq technique that utilizes custom droplet-based microfluidics and bulk reverse transcription. Highly parallel genome-wide expression profiling of individual cells using nanoliter droplets. The spread of next-generation sequencing (NGS) platforms has further improved the potential of this technique, speeding up the possibility of simultaneously analysing multiple ingredients from. A research article presenting a high-throughput single-cell RNA sequencing (scRNA-seq) technique that utilizes custom droplet-based microfluidics and in-droplet reverse transcription. Microbial identification using 16S rDNA variable regions has become increasingly popular over the past decade. Droplet barcoding for single-cell transcriptomics applied to embryonic stem cells. ![]() More information: Floris Breman – Royal Museum for Central Africa – and on. Registration: Registration was free (starting at 12:00) but mandatory (in order to arrange the coffee breaks). Koen De Gelas ( Ecology, Evolution and Biodiversity Conservation Section, KULeuven & Royal Belgian Institute for Natural Sciences): Aspects of amplicon sequencing using NGS.ġ7:15-17:30 Discussion and closure (Moderator: Dr. Carl Van Gestel ( Entomology Department, Royal Belgian Institute of Natural Sciences): Applying NGS for exploring adaptive genetic variation in non-model organisms.ġ6:15-1700 Dr. Guus Roeselers ( TNO Netherlands, Microbiology & Systems Biology): Probing diversity in a hidden world: applications of NGS in microbial ecology.ġ5:15-16:00 Dr. Jeroen Van Houdt (Genomics Core KULeuven – UZLeuven): New sequencing technologies: possibilities and limitations for archival DNA studies.ġ4:00-14:45 Dr. ![]() Cybertrucks soon to be sold out for ‘24, but Tesla reports 15 decline in gross profit for ’23. Marc De Meyer (Head of the entomology section of the Royal Museum for Central Africa and Coordinator of JEMU).ġ3:00-13:45 Dr. Next-Gen Teslas Come from Texas in 2025, Musk Says. This work introduces a more effective multi-locus barcoding framework that is based on gene capture and next-generation sequencing and predicts that, as the cost of DNA sequencing continues to fall, it will eclipse existing single- locus DNA barc coding methods as a means to better understand the diversity of the living world. Program (with links to the pdf files of the presentations):ġ2:45 opening of the seminar by Dr. ![]() ![]() Main topics: general introduction to next generation sequencing (NGS), case studies and specific applications (final program here). Symposium venue: Royal Museum for Central Africa - Leuvensesteenweg 17 – B-3080 Tervuren. The Joint Experimental Molecular Unit (JEMU) of the Royal Belgian Institute of Natural Sciences (RBINS) and of the Royal Museum for Central Africa (RMCA) organised a half-day symposium on next generation sequencing and its applications in science.ĭate: Monday 21 October 2013, 13:00-17:00 In this study, we newly generated 237 plastomes of 50 species (at least two individuals per species) by genome skimming, covering 71. ![]()
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